2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Nisbiy molekulyar massa taqsimotining kalibrlash egri chizig'ida ishlatiladigan standart moddalar: insulin, mikopeptidlar, glisin-glisin-tirozin-arginin, glisin-glisin-glisin
3 Asboblar va uskunalar
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Umuman olganda, Sustar mahsulotlaridagi aminokislotalarning ulushi Zinpro mahsulotlaridagiga qaraganda yuqori.
8-qism Foydalanishning ta'siri
Tuxum qo'yishning oxirgi davrida tuxum qo'yadigan tovuqlarning ishlab chiqarish ko'rsatkichlari va tuxum sifatiga turli xil mikroelementlar manbalarining ta'siri
Ishlab chiqarish jarayoni
Maqsadli xelatlash texnologiyasi
Qirqim emulsifikatsiyasi texnologiyasi
Bosimli purkash va quritish texnologiyasi
Sovutish va namlikni yo'qotish texnologiyasi
Ilg'or atrof-muhitni boshqarish texnologiyasi
A ilova: Peptidlarning nisbiy molekulyar massa taqsimotini aniqlash usullari
Standartni qabul qilish: GB/T 22492-2008
1 Sinov printsipi:
Bu yuqori samarali gel filtrlash xromatografiyasi yordamida aniqlandi. Ya'ni, statsionar faza sifatida g'ovakli plomba moddasidan foydalanib, ajratish uchun namunaviy komponentlarning nisbiy molekulyar massa o'lchamidagi farqga asoslanib, 220 nm ultrabinafsha yutilish to'lqin uzunligining peptid bog'lanishida aniqlangan holda, gel filtrlash xromatografiyasi (ya'ni, GPC dasturi) yordamida nisbiy molekulyar massa taqsimotini aniqlash uchun maxsus ma'lumotlarni qayta ishlash dasturidan foydalanib, xromatogrammalar va ularning ma'lumotlari qayta ishlandi, soya peptidining nisbiy molekulyar massasi va taqsimot diapazoni hajmini olish uchun hisoblab chiqildi.
2. Reaktivlar
Tajriba suvi GB/T6682 dagi ikkilamchi suvning spetsifikatsiyasiga javob berishi kerak, maxsus qoidalar bundan mustasno, reagentlardan foydalanish analitik jihatdan toza bo'lishi kerak.
2.1 Reaktivlar tarkibiga asetonitril (xromatografik jihatdan sof), triftorsirka kislotasi (xromatografik jihatdan sof) kiradi.
2.2 Nisbiy molekulyar massa taqsimotining kalibrlash egri chizig'ida ishlatiladigan standart moddalar: insulin, mikopeptidlar, glisin-glisin-tirozin-arginin, glisin-glisin-glisin
3 Asboblar va uskunalar
3.1 Yuqori samarali suyuq xromatografiya (HPLC): UV detektori va GPC ma'lumotlarni qayta ishlash dasturiga ega xromatografik ish stantsiyasi yoki integrator.
3.2 Ko'chma fazali vakuumli filtrlash va gazsizlantirish bloki.
3.3 Elektron balans: gradusli qiymat 0.000 1g.
4 Ishlash bosqichlari
4.1 Xromatografik sharoitlar va tizimga moslashish tajribalari (mos yozuvlar shartlari)
- 4.1.1 Xromatografik ustun: TSKgelG2000swxl300 mm×7.8 mm (ichki diametri) yoki oqsillar va peptidlarni aniqlash uchun mos keladigan, o'xshash ishlashga ega bo'lgan bir xil turdagi boshqa gel ustunlari.
- 4.1.2 Ko'chma faza: Asetonitril + suv + triftorsirka kislotasi = 20 + 80 + 0.1.
- 4.1.3 Aniqlash to'lqin uzunligi: 220 nm.
- 4.1.4 Oqim tezligi: 0,5 ml/min.
- 4.1.5 Aniqlash vaqti: 30 daqiqa.
- 4.1.6 Namuna in'ektsiyasi hajmi: 20 μL.
- 4.1.7 Ustun harorati: xona harorati.
- 4.1.8 Xromatografik tizimni aniqlash talablariga javob berishi uchun yuqoridagi xromatografik sharoitlarda gel xromatografik ustunining samaradorligi, ya'ni nazariy plastinkalar soni (N) tripeptid standartining (Glisin-Glisin-Glisin) cho'qqilari asosida hisoblanganda 10000 dan kam bo'lmasligi shart qilib qo'yildi.
- 4.2 Nisbiy molekulyar massa standart egri chiziqlarini hosil qilish
- Yuqorida keltirilgan 1 mg/ml massa konsentratsiyasiga ega bo'lgan turli xil nisbiy molekulyar massali peptid standart eritmalari mobil faza moslashuvi orqali tayyorlandi, ma'lum bir nisbatda aralashtirildi va keyin 0,2 mkm ~ 0,5 mkm teshik o'lchamiga ega organik faza membranasi orqali filtrlandi va namunaga kiritildi, so'ngra standartlarning xromatogrammalari olindi. Nisbiy molekulyar massa kalibrlash egri chiziqlari va ularning tenglamalari nisbiy molekulyar massa logarifmini saqlash vaqtiga nisbatan chizish yoki chiziqli regressiya orqali olindi.
4.3 Namuna bilan ishlash
10 ml hajmli kolbada 10 mg namunani aniq torting, ozgina mobil faza qo'shing, 10 daqiqa davomida ultratovush chayqating, shunda namuna to'liq eriydi va aralashtiriladi, mobil faza bilan tarozigacha suyultiriladi va keyin 0,2 μm ~ 0,5 μm teshik o'lchamiga ega organik faza membranasi orqali filtrlanadi va filtrat A.4.1 dagi xromatografik sharoitlarga muvofiq tahlil qilinadi.
- 5. Nisbiy molekulyar massa taqsimotini hisoblash
- 4.1 xromatografik sharoitida 4.3 da tayyorlangan namunaviy eritmani tahlil qilgandan so'ng, namunaning nisbiy molekulyar massasi va uning tarqalish diapazoni namunaning xromatografik ma'lumotlarini GPC ma'lumotlarini qayta ishlash dasturi yordamida 4.2 kalibrlash egri chizig'iga qo'yish orqali olinishi mumkin. Turli peptidlarning nisbiy molekulyar massalarining taqsimotini quyidagi formula bo'yicha cho'qqi maydonini normallashtirish usuli bilan hisoblash mumkin: X=A/A jami × 100
- Formuladagi formula: X - Namunadagi umumiy peptiddagi nisbiy molekulyar massa peptidining massa ulushi, %;
- A - Nisbiy molekulyar massali peptidning cho'qqi maydoni;
- Jami A - har bir nisbiy molekulyar massa peptidining cho'qqi maydonlarining yig'indisi, bitta kasrgacha hisoblanadi.
- 6 Takrorlanish
- Takrorlanish shartlari ostida olingan ikkita mustaqil aniqlash orasidagi mutlaq farq ikki aniqlashning arifmetik o'rtacha qiymatining 15% dan oshmasligi kerak.
- B ilova: Erkin aminokislotalarni aniqlash usullari
- Standartni qabul qilish: Q/320205 KAVN05-2016
- 1.2 Reaktivlar va materiallar
- Muzlik sirka kislotasi: analitik jihatdan sof
- Perxlorid kislotasi: 0,0500 mol/L
- Indikator: 0,1% kristall binafsha indikator (muzlik sirka kislotasi)
- 2. Erkin aminokislotalarni aniqlash
Namunalar 80°C da 1 soat davomida quritildi.
Namunani xona haroratiga qadar tabiiy ravishda sovutish yoki foydalanishga yaroqli haroratgacha sovutish uchun quruq idishga joylashtiring.Taxminan 0,1 g namunani (0,001 g gacha aniqlikda) 250 ml quruq konussimon kolbaga soling.Namuna atrof-muhit namligini yutib yubormasligi uchun tezda keyingi bosqichga o'ting25 ml muz sirka kislotasini qo'shing va 5 daqiqadan ko'p bo'lmagan vaqt davomida yaxshilab aralashtiring.2 tomchi kristall binafsha indikator qo'shingEritma binafsha rangdan oxirgi nuqtaga o'zgarguncha 0,0500 mol/L (±0,001) standart xlorid kislotasi titrlash eritmasi bilan titrlang.
Iste'mol qilingan standart eritma hajmini yozib oling.
- Bo'sh testni bir vaqtning o'zida bajaring.
- 3. Hisoblash va natijalar
- Reagentdagi erkin aminokislota miqdori X massa ulushi (%) sifatida ifodalanadi va quyidagi formula bo'yicha hisoblanadi: X = C × (V1-V0) × 0.1445/M × 100%, quyidagi formula bo'yicha:
- C - Standart perxlorid kislota eritmasining litrdagi mollarda konsentratsiyasi (mol/L)
- V1 - Namunalarni standart perxlorik kislota eritmasi bilan titrlash uchun ishlatiladigan hajm, millilitrlarda (ml).
- Vo - standart perxlorid kislota eritmasi bilan titrlash uchun ishlatiladigan hajm, millilitrlarda (ml);
M - Namunaning massasi, grammda (g).
| 0.1445: 1.00 ml standart xlorid kislota eritmasiga teng aminokislotalarning o'rtacha massasi [c (HClO4) = 1.000 mol / L]. | 4.2.3 Seriy sulfat standart titrlash eritmasi: konsentratsiya c [Ce (SO4) 2] = 0,1 mol/L, GB/T601 ga muvofiq tayyorlangan. | |
| Standartlarni qabul qilish: Q/70920556 71-2024 | 1. Aniqlash printsipi (misol sifatida Fe) | Aminokislota temir komplekslari suvsiz etanolda juda past eruvchanlikka ega va erkin metall ionlari suvsiz etanolda eriydi, aminokislota temir komplekslarining xelatlanish tezligini aniqlash uchun suvsiz etanolda ikkalasi o'rtasidagi eruvchanlik farqi ishlatilgan. |
| Formuladagi formula: V1 - sinov eritmasini titrlash uchun sarflangan seriy sulfat standart eritmasining hajmi, ml; | Suvsiz etanol; qolgan qismi GB/T 27983-2011 dagi 4.5.2-band bilan bir xil. | 3. Tahlil bosqichlari |
| Parallel ravishda ikkita sinovni bajaring. 103±2℃ da 1 soat davomida quritilgan namunadan 0,1 g ni torting, 0,0001 g gacha aniqlikda torting, 100 ml suvsiz etanol qo'shing, eritiladi, filtrlang, 100 ml suvsiz etanol bilan yuvilgan qoldiqni kamida uch marta filtrlang, so'ngra qoldiqni 250 ml konussimon kolbaga soling, GB/T27983-2011 ning 4.5.3-bandiga muvofiq 10 ml sulfat kislota eritmasini qo'shing va keyin GB/T27983-2011 ning 4.5.3-bandiga muvofiq "Eritguncha qizdiring va keyin sovushini kuting" ga muvofiq quyidagi amallarni bajaring. Bo'sh sinovni bir vaqtning o'zida o'tkazing. | 4. Umumiy temir miqdorini aniqlash | 4.1 Aniqlash tamoyili GB/T 21996-2008 ning 4.4.1-bandi bilan bir xil. |
4.2. Reaktivlar va eritmalar
| 4.2.1 Aralash kislota: 700 ml suvga 150 ml sulfat kislota va 150 ml fosfor kislotasini qo'shing va yaxshilab aralashtiring. | 4.2.2 Natriy difenilamin sulfonat indikator eritmasi: 5 g/L, GB/T603 ga muvofiq tayyorlangan. | 4.2.3 Seriy sulfat standart titrlash eritmasi: konsentratsiya c [Ce (SO4) 2] = 0,1 mol/L, GB/T601 ga muvofiq tayyorlangan. | |
| 4.3 Tahlil bosqichlari | Parallel ravishda ikkita sinovni o'tkazing. 0,1 g namunani 020001 g gacha aniqlikda torting, 250 ml konussimon kolbaga soling, 10 ml aralash kislota qo'shing, erigandan so'ng, 30 ml suv va 4 tomchi natriy dianilin sulfonat indikator eritmasini qo'shing va keyin GB/T21996-2008 ning 4.4.2-bandiga muvofiq quyidagi amallarni bajaring. Bo'sh sinovni bir vaqtning o'zida o'tkazing. | 4.4 Natijalarning ifodalanishi | Aminokislota temir komplekslarining umumiy temir miqdori X1 temirning massa ulushi bo'yicha, qiymati % da ifodalangan holda (1) formula bo'yicha hisoblab chiqilgan: |
| X1=(V-V0)×C×M×10-3×100 | V0 - bo'sh eritmani titrlash uchun sarflangan seriy sulfat standart eritmasi, ml; | V0 - bo'sh eritmani titrlash uchun sarflangan seriy sulfat standart eritmasi, ml; | C - Seriy sulfat standart eritmasining haqiqiy konsentratsiyasi, mol/L5. Xelatlardagi temir miqdorini hisoblashXelat tarkibidagi temirning massa ulushi bo'yicha X2 miqdori, % da ifodalangan qiymati quyidagi formula bo'yicha hisoblab chiqilgan: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Formuladagi formula: V1 - sinov eritmasini titrlash uchun sarflangan seriy sulfat standart eritmasining hajmi, ml; | V2 - bo'sh eritmani titrlash uchun sarflangan seriy sulfat standart eritmasi, ml;nom1 - Namuna massasi, g. Parallel aniqlash natijalarining arifmetik o'rtacha qiymatini aniqlash natijalari sifatida oling va parallel aniqlash natijalarining absolyut farqi 0,3% dan oshmaydi. | 0,05585 - 1,00 ml seriy sulfat standart eritmasi C[Ce(SO4)2.4H20] ga teng bo'lgan grammda ifodalangan temir temirning massasi = 1,000 mol/L.nom1 - Namuna massasi, g. Parallel aniqlash natijalarining arifmetik o'rtacha qiymatini aniqlash natijalari sifatida oling va parallel aniqlash natijalarining absolyut farqi 0,3% dan oshmaydi. | 6. Xelatsiya tezligini hisoblashXelatatsiya tezligi X3, qiymati % da ifodalangan X3 = X2/X1 × 100C ilova: Zinpro xelatlanish tezligini aniqlash usullari |
Standartni qabul qilish: Q/320205 KAVNO7-2016
1. Reaktivlar va materiallar
a) Muzlik sirka kislotasi: analitik jihatdan sof; b) Perxlorid kislotasi: 0,0500 mol/L; c) Indikator: 0,1% kristall binafsha indikator (muzlik sirka kislotasi)
2. Erkin aminokislotalarni aniqlash
2.1 Namunalar 80°C da 1 soat davomida quritildi.
2.2 Namunani xona haroratiga qadar tabiiy ravishda sovutish yoki foydalanishga yaroqli haroratgacha sovutish uchun quruq idishga soling.
2.3 Taxminan 0,1 g namunani (0,001 g gacha aniqlikda) 250 ml quruq konussimon kolbaga soling.
2.4 Namuna atrof-muhit namligini yutib yubormasligi uchun tezda keyingi bosqichga o'ting.
2.5 25 ml muz sirka kislotasini qo'shing va 5 daqiqadan ko'p bo'lmagan vaqt davomida yaxshilab aralashtiring.
2.6 2 tomchi kristall binafsha indikator qo'shing.
2.7 0,0500 mol/L (±0,001) standart xlorid kislotasi titrlash eritmasi bilan eritma binafsha rangdan yashil rangga o'zgarguncha 15 soniya davomida titrlang, natijada rangi o'zgarmaydi.
2.8 Iste'mol qilingan standart eritma hajmini yozib oling.
2.9 Bo'sh testni bir vaqtning o'zida bajaring.
- 3. Hisoblash va natijalar
- Katalancha
- Physicochemical parameters
V1 - Namunalarni standart perxlorik kislota eritmasi bilan titrlash uchun ishlatiladigan hajm, millilitrlarda (ml).
Vo - standart perxlorid kislota eritmasi bilan titrlash uchun ishlatiladigan hajm, millilitrlarda (ml);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Manzil: Xitoy, Sichuan viloyati, Chengdu shahri, Pujiang okrugi, Shouan shahri, Qingpu yo'li, 147-uy
Telefon: 86-18880477902
Mahsulotlar
Noorganik iz minerallari
- Organik iz minerallari
- Suaxili tili
- Moslashtirilgan xizmat
- Tezkor havolalar
Kompaniya profili
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | So'rov uchun bosing | © Mualliflik huquqi - 2010-2025: Barcha huquqlar himoyalangan. | Sayt xaritasi ENG YAXSHI QIDIRUV Telefon |
| Tel | 86-18880477902 | Yavacha | Elektron pochta |
| 8618880477902 | Xitoycha | Fransuzcha | |
| Bird | Xitoycha | Fransuzcha | Nemischa Ispancha |
| Aquatic animals | Yaponcha | Koreyscha | Arabcha Yunoncha |
| Turkcha | Italiyacha | ||
| Ruminant animal g/head day | January 0.75 | Indonez tili Afrikaans Shvedcha |
Polsha
- Baskcha
- Katalancha
- Physicochemical parameters
Hind tili
Laoscha
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bolgarcha
- Sebuancha
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Xorvatcha
Gollandiyalik
| Application object | Urducha Vetnamcha | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Gaiticha | Xausa | Kinyarvanda Hmong Vengercha |
| Piglets and fattening pigs | Igbo | Yavacha | Kannada Khmer Kurdcha |
| Qirg'iz | Lotin tili | ||
| Bird | 300～400 | 45～60 | Makedoniyalik Malaycha Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norvegcha
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbcha
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Suaxili tili
Tojikcha
Tamilcha
Telugu
Taylandcha
| Application object | Urducha Vetnamcha | Content in full-value feed (mg/kg) | Efficacy |
| Yidishcha | Yoruba | Zulu | Kinyarvanda Oriya Turkman |
| Uyg'ur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
